Isolation of (copper, zinc)-thioneins from the livers of copper-injected rats.

نویسندگان

  • I Bremner
  • B W Young
چکیده

The mixed copper- and zinc-binding proteins, with mol.wt. of approx. 12000, induced in rat liver after injection of copper were isolated and characterized as metallothioneins. Three separate forms were obtained, with 7-11% total metal in the protein, equivalent to 2.6-1.6 cysteine residues/metal atom.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Paradoxical Regulation of Copper and Zinc and Changes in Neurogenesis, Alcohol Preference and Salt Appetite in Isolated Male Rats

Background and Aims: Alcohol abuse is an important concern of many societies. Hippocampal neurogenesis regulates abusing drugs in a positive manner. The aim of this study was to identify factors that regulate neurogenesis in isolation period that increase preference for alcohol and salt. Materials and Methods: In this study sixteen rats were randomly divided into two groups: Pair (social) an...

متن کامل

Studies on the Appearance of a Copper-Binding Protein in Rat Liver

Studies on metalloproteins in ruminant livers showed the relationship between the total amount of zinc and copper present in a metallothionein-like fraction (designated fraction 111) and the zinc content of livers can be described by the equation y = 0.74~-0.25 (s.E. of regression coefficient = h0.04, P<O.OOl), where y is the number of g-atoms of Cu+Zn in fraction III/g of fresh liver and x is ...

متن کامل

Modulation of Fourier Transform Infrared Spectra and Copper Levels by Purslane (Portulaca Oleracea) Against Liver Necrosis Induced by Copper Sulphate

Copper (Cu) is an essential trace element involved in normal reproduction but its over-exposure may produce some detrimental effects. The aim of this study was to investigate the effects of purslane on copper sulfate poisoning on liver structures changes. Twenty eight wistar rats were randomly allocated to four treatment groups. Group I) Control, Group II) Copper sulfate (200 mg/kg bw were appl...

متن کامل

Printed in Great Britain Effect of Zinc Status of Rats on the Synthesis and Degradation of Copper - hduced Metailothioneins

Injection ofZn2+-adequate and Zn2+-deficient rats with Cu2+ stimulated the incorporation of L-[35S]cysteine into a low-molecular-weight Cu2+-binding protein in both liver and kidney. No significant incorporation of L-[4,5-3H]leucine into this protein occurred, confirming the previous claim that it was metallothionein and not some other leucine-rich protein. The half-life of the protein was foun...

متن کامل

Cadmium , Zinc and Copper Accumulation in Limpets ( Patella vulgata ) from the Bristol Channel with Special Reference to Metallothioneins

The study of metal concentrations in Patella vulgata caught from a polluted environment reveals a direct relationship between Cd concentration and body size and an inverse relationship in regard to Zn and Cu. Most of the Cd present in limpets with heavy Cd loads is bound to thioneins, but this is not the case for Zn and Cu. In young limpets, Cd-thioneins were not detected. All results indicate ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • The Biochemical journal

دوره 157 2  شماره 

صفحات  -

تاریخ انتشار 1976